Identification, Isolation and Characterization of IGF Binding Proteins as a Secretable Proteins of Human Endometrial Stromal Cell Culture.

IGF-1, a high potent mitogenic factor with a dual function is believed to participate with female steroids and other growth factors to prepare endometrium receptivity for successful embryo implantation and further development. Stromal cells forms the functional receptive layer of endometrium structure. Analysis of the conditioned/defined medium of pure cultured human endometrial stromal cells have revealed that stromal cells in a monolayer culture secrete and produce three different types of proteins in a varying amounts. These proteins exhibit a high specificity as well as a high binding affinity for the radiolabeled IGF-1 peptide. The molecular weights of these proteins have been determined by SDS-Page electrophoresis after cross-linking with the radiolegand IGF-1 and then detected with autoradiography. By comparison to the migration of high molecular weights protein markers, these proteins have been identified to correspond to the IGFBP-1 (31 KDa), IGFBP-2 (36 KDa) and IGFBP-3 (45 KDa). The secretion of these binding proteins (IGFBP-1, -2, -3) by endometrial stromal cells may support the view of their biological importance in controlling the delivery and bioavailability of the high mitogen IGF-1 peptide to their nominative type-1 IGF-receptor on cell surface, thereby modulating its action. It seems likely that these IGFBPs may play a key role in switching on/off IGF-1 peptide action , thereby avoiding the uncontrolled proliferation effect of the IGF-1 that favor endometrium cancer development.

Green Tea Attenuates Experimental Hepatitis in Context of Oxidative Stress.

Food derived antioxidants have a strong potential for long term use as chemo-preventive agents in disease states involving oxidative stress such as tea. This study was done to clarify the potential effect of green tea extract in protecting the liver from lipopolysaccharide (LPS)-induced hepatitis in D-galactosamine (D-GalN) sensitized rats. Forty male albino rats weighing 180 - 200 g were divided into four groups; group I: healthy rats received vehicle only, group II: healthy rats received green tea (GT) extract orally for thirty days, Group III: healthy rats received vehicle only before induction of hepatitis. Group IV: healthy rats received green tea extract orally for thirty days before induction of hepatitis. After the experimental period, serum liver enzymes and liver oxidant /antioxidant profile were determined. Liver coenzymeQ10 (CoQ10) was estimated by HPLC method using C18 column and UV detector at 275 nm. The current date appeared the effective role of green tea extract in protecting the liver against the injury induced by D-GalN/LPS which may be attributed to polyphenolic compounds that scavenge a wide range of free radicals and increasing the antioxidant enzymes.